Journal: Methods in molecular biology (Clifton, N.J.)

Article Title: Chromatin Preparation and Chromatin Immuno-precipitation from Drosophila Embryos.

PMID/MRN: 27659972

Publication Date: 2016-09-23

Abstract:

This protocol provides specific details on how to perform Chromatin immunoprecipitation (ChIP) from Drosophila embryos. ChIP allows the matching of proteins or histone modifications to specific genomic regions. Formaldehyde-cross-linked chromatin is isolated and antibodies against the target of interest are used to determine whether the target is associated with a specific DNA sequence. This can be performed in spatial and temporal manner and it can provide information about the genome-wide localization of a given protein or histone modification if coupled with deep sequencing technology (ChIP-Seq).