Journal: Artificial cells, nanomedicine, and biotechnology
Article Title: One cell model establishment to inhibit CaMKII ? mRNA expression in the dorsal root ganglion neuron by RNA interfere.
PMID/MRN: 27685016
Publication Date: 2016-Aug-16
Abstract:
CaMKII ? in dorsal root ganglion neurons is closely related to the neuropathic pain, neuron injury induced by local anesthetics. To get great insight into the function of CaMKII ? in dorsal root ganglion neurons, we need one cell model to specially inhibit the CaMKII ? mRNA expression. The present study was aimed to establish one cell model to specially inhibit the CaMKII ? mRNA expression. We designed the CaMKII ? shRNA sequence and connected with pYr-1.1 plasmid. The ligation product of the CaMKII ? shRNA and pYr-1.1 plasmid was recombined with pAd/PL-DEST vector into pAD-CaMKII?-shRNA. adenovirus vector. pAD-CaMKII?-shRNA. adenovirus vector infected the dorsal root ganglion neuron to inhibit the CaMKII ? mRNA expression in vitro. The pAD-CaMKII?-shRNA adenovirus vector was verified to be correct by the digestion, sequence. And pAD-CaMKII?-shRNA. adenovirus vector can infect the DRG cells to inhibit the CaMKII ? mRNA or protein expression by the real-time polymerase chain reaction (PCR) or western blotting. Those results showed that we successfully constructed one adenovirus vector that can infect the dorsal root ganglion neuron to inhibit the CaMKII ? mRNA and protein expression. That will supply with one cell model for the CaMKII ? function study.