Journal: Methods in molecular biology (Clifton, N.J.)
Article Title: Characterizing ErbB-2-Mediated Tyrosine Phosphorylation and Activation of Plexins.
PMID/MRN: 27787847
Publication Date: 2017-10-28
Abstract:
Plexins comprise a family of transmembrane receptors for semaphorins. Plexins of the B- and D-subfamily interact with the receptor tyrosine kinase ErbB-2, and this interaction has been shown to be functionally relevant for various biological processes including tumor metastasis and bone formation. Binding of semaphorins to B- and D-subfamily plexins results in the activation of ErbB-2, which in turn phosphorylates these plexins. This phosphorylation triggers the activation of the small GTPases RhoA and RhoC downstream of B-subfamily plexins. Here we describe a methodology that allows the analysis of ErbB-2-mediated plexin phosphorylation and signaling.