Journal: International journal of biological macromolecules
Article Title: Structural and thermal characterization of PHAs produced by Lysinibacillus sp. through submerged fermentation process.
PMID/MRN: 27667539
Publication Date: 2016-Dec-05
Abstract:
In this investigation an attempt has been made to characterize and identify Lysinibacillus sp. 3HHX by 16S-rDNA sequencing. The bacterium exhibited occurrence of PHAs granules on an average 11±1 per cell of 1.0?m length and breadth 0.72?m, revealed from TEM studies. Under optimized condition, 4.006gm/L of PHAs was extracted using hypochlorite digestion and multi-solvent extraction process. PhaC gene of ~540bp and higher PHA synthase activity was detected at 48h of cultivation. The extracted PHAs was structurally characterized by GC-MS and 1 H NMR reported to be P(3HB-co-3HDD-co-3HTD) and amorphous in nature with 112ĄC melting point, -11.0ĄC glass transition point and 114.76ĄC decomposition temperature detected by DSC & TGA respectively. The C/O of biopolymer disc was 1:65 as revealed from C1s and O1s spectra of XPS, that was completely biodegradable within 30 days. This biopolymer was observed to be non-cytotoxic to NIH 3T3 mouse fibroblast cells. The report is of its kind in establishing the abilities of Lysinibacillus sp. 3HHX for non-growth associated PHA co-polymer production. Moreover the biocompatible and biodegradable nature of the biopolymer conferred to its substantial biomedical applications.
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